Humanely raised and safely sourced free-range chicken raised without antibiotics, artificial growth hormones, or steroids. The "Brushed on Benefits" treats were developed by Dr. Bob Goldstein. The Calm treats are delicious chicken breast cutlets brushed with Vitamin E and L-Tryptophanin a tasty olive oil marinade to help keep your pet calm and relaxed. This treat is free of wheat, corn, soy, or any other grain. Made and sourced in the USA. Manufactured in a USDA inspected human food facility.